Evaluation of microplate immunocapture method for detection of Vibrio cholerae, Salmonella Typhi and Shigella flexneri from food

Salmonella serotype Typhi Shigella Vibrio cholerae Bacterial Agents of Enteric Diseases of Public Health Concern

Reverse transcription-multiplex PCR assay for simultaneous detection of Escherichia coli O157:H7, Vibrio cholerae O1, and Salmonella Typhi.

BACKGROUND Escherichia coli O157:H7, Vibrio cholerae O1, and Salmonella Typhi are pathogenic bacteria that can be found in contaminated water supplies throughout the world. No currently available assays can simultaneously detect and identify all three pathogens. Our aim was to develop a rapid and reliable technique for simultaneous detection of these pathogens. METHODS Four unique genes were ...

Development and Application of an Immunocapture PCR Diagnostic Assay Based on the Monoclonal Antibody for the Detection of Shigella

Background: Shigella is among the most important human pathogenic microorganisms, infecting both humans and nonhuman and causing clinically severe diarrhea. Shigella must be enriched before detection, which is time-consuming.Objectives: To develop a sensitive, rapid, and specifc method for Shigella detection.Materials and Methods: S...

Isolation, Characterization and Determination of Antimicrobial Properties of Lactic Acid Bacteria from Human Milk

Breast milk has a distinctive combination of proteins, carbohydrates, minerals, lipids and various vitamins that promote the proper growth, development and immunity of the infants. That’s why it is considered to be a complete food for new born babies. Moreover, it is also rich in various bioactive compounds which promote the maturation of immune system as well as develop body’s defense against ...

Inactivation and sub-lethal injury of salmonella typhi, salmonella typhimurium and vibrio cholerae in copper water storage vessels

BACKGROUND This study provides information on the antibacterial effect of copper against the water-borne pathogens Salmonella Typhi, Salmonella Typhimurium and Vibrio cholerae. METHODS Suspensions of each pathogen were kept in water within a traditional copper vessel at 30°C for 24 h. Samples were withdrawn, diluted and plated onto suitable growth media. Conventional enumeration of healthy (u...